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rabbit anti sparcl1 (Bioss)

Name: rabbit anti sparcl1
Brand: Bioss
Rating: 94 (4 reviews)

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Bioss rabbit anti sparcl1
Rabbit Anti Sparcl1, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti sparcl1/product/Bioss
Average 94 stars, based on 4 article reviews

rabbit anti sparcl1 - by Bioz Stars, 2026-02

94/100 stars

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Secreted protein acidic and rich in cysteine like 1 (SPARCL1) interacts with integrin β1 (ITGB1). ( A ) Co-immunoprecipitation with an anti-SPARCL1 antibody followed by Western blotting with an anti-ITGB1 antibody. ( B ) Co-immunoprecipitation with an anti-ITGB1 antibody followed by Western blotting with an anti-SPARCL1 antibody. Input, IgG, and IP represent the positive control, negative control, and target experimental group, respectively.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: Secreted protein acidic and rich in cysteine like 1 (SPARCL1) interacts with integrin β1 (ITGB1). ( A ) Co-immunoprecipitation with an anti-SPARCL1 antibody followed by Western blotting with an anti-ITGB1 antibody. ( B ) Co-immunoprecipitation with an anti-ITGB1 antibody followed by Western blotting with an anti-SPARCL1 antibody. Input, IgG, and IP represent the positive control, negative control, and target experimental group, respectively.

Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R), rabbit anti-ARP2/3 subunit 1B antibody (bs-10563R), rabbit anti-CDC42 antibody (bs-3555R), rabbit anti-MyoD antibody (bs-2442R), rabbit anti-GAPDH antibody (bs-0755R) (all at dilution 1:500; Bioss Antibodies, China).

Techniques: Immunoprecipitation, Western Blot, Positive Control, Negative Control

SPARCL1 influences MDSC migration and differentiation. ( A , C ) Cell scratch assay images at 20 h after SPARCL1 activation or inhibition. ( B , D ) Quantification of the cell migration rate at 20 h after SPARCL1 activation or inhibition according to ( A , C ). ( E , G ) Desmin immunofluorescence staining in MDSCs after SPARCL1 activation or inhibition. ( F , H ) Quantification of the myotube rate based on ( E , G ). ( I , K ) Western blotting images of MyoD expression after SPARCL1 activation or inhibition. ( J , L ) Quantification of MyoD from Western blotting results presented in ( I , K ). Scale bar = 100 μm. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 influences MDSC migration and differentiation. ( A , C ) Cell scratch assay images at 20 h after SPARCL1 activation or inhibition. ( B , D ) Quantification of the cell migration rate at 20 h after SPARCL1 activation or inhibition according to ( A , C ). ( E , G ) Desmin immunofluorescence staining in MDSCs after SPARCL1 activation or inhibition. ( F , H ) Quantification of the myotube rate based on ( E , G ). ( I , K ) Western blotting images of MyoD expression after SPARCL1 activation or inhibition. ( J , L ) Quantification of MyoD from Western blotting results presented in ( I , K ). Scale bar = 100 μm. * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques: Migration, Wound Healing Assay, Activation Assay, Inhibition, Immunofluorescence, Staining, Western Blot, Expressing

SPARCL1 affects an ITGB1-mediated signaling pathway. ( A , I ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 after SPARCL1 activation or inhibition, respectively. ( B – H ) Quantification of the Western blotting results presented in ( A ). ( J – P ) Quantification of the Western blotting results presented in ( I ). ** p < 0.01.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 affects an ITGB1-mediated signaling pathway. ( A , I ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 after SPARCL1 activation or inhibition, respectively. ( B – H ) Quantification of the Western blotting results presented in ( A ). ( J – P ) Quantification of the Western blotting results presented in ( I ). ** p < 0.01.

Techniques: Western Blot, Activation Assay, Inhibition

SPARCL1 influences cell migration and differentiation through ITGB1. ( A ) Cell scratch assay images after simultaneous SPARCL1 activation and ITGB1 inhibition. ( B ) Quantification of the cell migration rate based on ( A ). ( C ) Desmin immunofluorescence staining of MDSCs after SPARCL1 activation and ITGB1 inhibition. ( D ) Quantification of the myotube rate represented in ( C ). ( E ) Western blotting results of MyoD expression after SPARCL1 activation and ITGB1 inhibition. ( F ) Quantification of the MyoD Western blotting results presented in ( E ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001; scale bar = 100 μm.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 influences cell migration and differentiation through ITGB1. ( A ) Cell scratch assay images after simultaneous SPARCL1 activation and ITGB1 inhibition. ( B ) Quantification of the cell migration rate based on ( A ). ( C ) Desmin immunofluorescence staining of MDSCs after SPARCL1 activation and ITGB1 inhibition. ( D ) Quantification of the myotube rate represented in ( C ). ( E ) Western blotting results of MyoD expression after SPARCL1 activation and ITGB1 inhibition. ( F ) Quantification of the MyoD Western blotting results presented in ( E ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001; scale bar = 100 μm.

Techniques: Migration, Wound Healing Assay, Activation Assay, Inhibition, Immunofluorescence, Staining, Western Blot, Expressing, Negative Control

SPARCL1 regulates an ITGB1-mediated signaling pathway through ITGB1. ( A ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 expression after SPARCL1 activation and ITGB1 inhibition. ( B – H ) Quantification of the Western blotting results presented in ( A ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 regulates an ITGB1-mediated signaling pathway through ITGB1. ( A ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 expression after SPARCL1 activation and ITGB1 inhibition. ( B – H ) Quantification of the Western blotting results presented in ( A ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques: Western Blot, Expressing, Activation Assay, Inhibition, Negative Control

Proposed model for SPARCL1 regulation of bovine MDSC migration and differentiation through ITGB1. As an extracellular matrix protein, SPARCL1 interacts with the transmembrane receptor ITGB1 and regulates changes in the expression of adhesion-plaque-associated proteins, such as ITGB1, p-FAK, FAK, p-paxillin, paxillin, and vinculin to affect the formation of focal adhesions. The Cdc42 and Arp2/3 complex was also affected by SPARCL1 through ITGB1. Further, bovine MDSC migration was stimulated by SPARCL1, and cells gathered and apparently expressed MyoD to begin an early-stage differentiation.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: Proposed model for SPARCL1 regulation of bovine MDSC migration and differentiation through ITGB1. As an extracellular matrix protein, SPARCL1 interacts with the transmembrane receptor ITGB1 and regulates changes in the expression of adhesion-plaque-associated proteins, such as ITGB1, p-FAK, FAK, p-paxillin, paxillin, and vinculin to affect the formation of focal adhesions. The Cdc42 and Arp2/3 complex was also affected by SPARCL1 through ITGB1. Further, bovine MDSC migration was stimulated by SPARCL1, and cells gathered and apparently expressed MyoD to begin an early-stage differentiation.

Techniques: Migration, Expressing

Hevin expression is altered at postnatal day (P) 14 in the cortex of Fmr1 knock-out (KO) mice. (A) Cultured cortical astrocytes co-labeled with anti-glial fibrillary acidic protein (GFAP; green) and anti-hevin (red) after 2 days in vitro . Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). Images were obtained using a 40x objective with a Zeiss Axioimager M2. Scale bars = 50 μm. (B) Representative western blots showing hevin (~130 kDa) in cortical samples (30 μg of protein per lane) from P7, P14 and P21 WT and Fmr1 KO mice, as well as the corresponding total protein within each lane. Negative controls that were run using P14 WT whole cortical tissue with either no primary antibody or no secondary antibody are shown. (C–E) Hevin expression in the cortex of WT (black; n = 8) and Fmr1 KO (white; n = 8) mice at P7, P14 and P21. Bands representing hevin were normalized against the total protein within the same lane on the membrane, and were then expressed as a percent of the average level of hevin in the WT group. (F) Hevin expression in cortical astrocytes isolated from P14 WT (black; n = 4) and Fmr1 KO (white; n = 4) mice. Immediately to the left of the graph is shown a representative Western blot with bands corresponding to hevin from P14 WT and Fmr1 KO cortical astrocyte samples (10 μg of protein per lane), as well as the corresponding total protein. Statistical differences were denoted with either a single asterisk, P < 0.05, or a double asterisks, P < 0.01.

Journal: Frontiers in Molecular Neuroscience

Article Title: Altered Developmental Expression of the Astrocyte-Secreted Factors Hevin and SPARC in the Fragile X Mouse Model

doi: 10.3389/fnmol.2017.00268

Figure Lengend Snippet: Hevin expression is altered at postnatal day (P) 14 in the cortex of Fmr1 knock-out (KO) mice. (A) Cultured cortical astrocytes co-labeled with anti-glial fibrillary acidic protein (GFAP; green) and anti-hevin (red) after 2 days in vitro . Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). Images were obtained using a 40x objective with a Zeiss Axioimager M2. Scale bars = 50 μm. (B) Representative western blots showing hevin (~130 kDa) in cortical samples (30 μg of protein per lane) from P7, P14 and P21 WT and Fmr1 KO mice, as well as the corresponding total protein within each lane. Negative controls that were run using P14 WT whole cortical tissue with either no primary antibody or no secondary antibody are shown. (C–E) Hevin expression in the cortex of WT (black; n = 8) and Fmr1 KO (white; n = 8) mice at P7, P14 and P21. Bands representing hevin were normalized against the total protein within the same lane on the membrane, and were then expressed as a percent of the average level of hevin in the WT group. (F) Hevin expression in cortical astrocytes isolated from P14 WT (black; n = 4) and Fmr1 KO (white; n = 4) mice. Immediately to the left of the graph is shown a representative Western blot with bands corresponding to hevin from P14 WT and Fmr1 KO cortical astrocyte samples (10 μg of protein per lane), as well as the corresponding total protein. Statistical differences were denoted with either a single asterisk, P < 0.05, or a double asterisks, P < 0.01.

Article Snippet: The following antibodies were used: rabbit anti-glial fibrillary acidic protein (GFAP; 1:500; catalog#: Z0334; Dako, Burlington, ON, Canada), chicken anti-GFAP (1:2000; catalog#: CH22102; Neuromics, Minneapolis, MN, USA) rabbit anti-hevin antibody (1:100; catalog#: bs-6110R; Bioss, Woburn, MA, USA), goat anti-SPARC antibody (10 μg/mL; catalog#: AF942; R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Knock-Out, Cell Culture, Labeling, In Vitro, Staining, Western Blot, Isolation

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